Alexandria Digital Research Library

Real-time measurement, analysis, and control in microfluidic systems for personalized medicine and designer materials

Author:
Mage, Peter L.
Degree Grantor:
University of California, Santa Barbara. Materials
Degree Supervisor:
Hyongsok T. Soh
Place of Publication:
[Santa Barbara, Calif.]
Publisher:
University of California, Santa Barbara
Creation Date:
2016
Issued Date:
2016
Topics:
Materials science and Biomedical engineering
Keywords:
Feedback control
Aptamer
FACS
Biosensors
Microfluidics
Genres:
Dissertations, Academic and Online resources
Dissertation:
Ph.D.--University of California, Santa Barbara, 2016
Description:

The field of microfluidics has enabled the development of powerful tools for analyzing and manipulating phenomena at the micro- and nano-scales, ranging from chemical analysis of biological samples to controlled synthesis of colloidal materials. In this dissertation we explore four unique platforms for real-time microfluidic measurement, analysis, and control systems with applications at the intersection of biomedicine and materials engineering. First, we show that a real-time biosensor can be used to perform closed-loop control of drug concentrations in the bloodstream of live animals. Second, we show that a commercially available cell-sorting instrument can be used to sort heterogeneous suspensions of synthetic microparticles based on shape using optical scattering measurements, resulting in monodisperse microparticle suspensions with well-defined morphology. Third, we report preliminary results for an image-based cell and microparticle sorter capable of sorting objects using two-dimensional high-speed microscopy and real-time image analysis. Finally, we report a contamination-resistant microfluidic assay for quantitative genetic detection based on real-time loop-mediated isothermal amplification, improving the robustness of point-of-care pathogen detection techniques.

Physical Description:
1 online resource (150 pages)
Format:
Text
Collection(s):
UCSB electronic theses and dissertations
ARK:
ark:/48907/f3qv3mb0
ISBN:
9781339671840
Catalog System Number:
990046534530203776
Rights:
Inc.icon only.dark In Copyright
Copyright Holder:
Peter Mage
File Description
Access: Public access
Mage_ucsb_0035D_12943.pdf pdf (Portable Document Format)