Alexandria Digital Research Library

Dynamic Imaging of Function and Morphogenesis in the Developing Heart

Ohn, Jungho
Degree Grantor:
University of California, Santa Barbara. Electrical & Computer Engineering
Degree Supervisor:
Michael Liebling
Place of Publication:
[Santa Barbara, Calif.]
University of California, Santa Barbara
Creation Date:
Issued Date:
Engineering, General, Engineering, Biomedical, and Health Sciences, Human Development
Dissertations, Academic and Online resources
Ph.D.--University of California, Santa Barbara, 2013

Congenital heart disease, the most common birth defect, occurs up to 5% of live births in humans. Understanding the relationship between morphology and function during development in the embryonic heart has important medical implications. Our studies focus on developing non-invasive heart imaging tools to observe the dynamics of morphogenesis while the heart is growing and functioning. Our approach consists in setting up a computer-driven automated microscope equipped with high-speed and sensitive cameras, implementing sample mounting protocols, and developing image reconstruction and analysis software. In order to improve the specificity with which structures can be imaged in the beating heart, we developed multi-modal microscopy by combining high-speed and multi-color sequences taken in brightfield and fluorescence microscopy, respectively. In an effort to improve axial resolution in conventional widefield microscopy, we implemented dynamic optical projection tomography. To quantify variability when characterizing heart function, we developed in vivo high-throughput microscopy. Finally, we carried out joint and quantitative studies of heart morphogenesis and functiogenesis by use of a novel imaging technique, burst time-lapse microscopy (BTLM). This allowed building time-lapses revealing the morphogenesis of the highly dynamic cardiac valve leaflets as they progressively prevent retrograde flow between cardiac chambers. This work provides new insights into cardiogenesis and should allow better understanding of normal and diseased development. We anticipate that the developed tools will be applicable for imaging a wide range of dynamic biological samples.

Physical Description:
1 online resource (136 pages)
UCSB electronic theses and dissertations
Catalog System Number:
Inc.icon only.dark In Copyright
Copyright Holder:
Jungho Ohn
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