Alexandria Digital Research Library

Identification and functional analysis of ABI4-interacting proteins in Arabidopsis

Author:
Swentowsky, Kyle William
Degree Grantor:
University of California, Santa Barbara. Molecular, Cellular & Developmental Biology
Degree Supervisor:
Ruth R. Finkelstein
Place of Publication:
[Santa Barbara, Calif.]
Publisher:
University of California, Santa Barbara
Creation Date:
2016
Issued Date:
2016
Topics:
Molecular biology and Plant sciences
Keywords:
ABI4
Arabidopsis
Abscisic Acid
Abiotic stress
ZFP2
ABA
Genres:
Dissertations, Academic and Online resources
Dissertation:
M.A.--University of California, Santa Barbara, 2016
Description:

Land plants utilize the hormone abscisic acid (ABA) to cope with drought, salt, and other abiotic stresses. ABA-INSENSITIVE4 (ABI4) is a transcription factor central to the ABA response in Arabidopsis thaliana but there are still many questions surrounding its regulation and transcriptional activity which might be explained by understanding its interacting partners. Here, we present data from a next generation sequencing yeast two-hybrid (NGS-Y2H) screen used to discover ABI4-interacting proteins. Using additional yeast two-hybrid and split-YFP experiments, we were able to confirm an ABI4 interaction with DWA1, DWA2, ZFP2, and eIF3f. Although the E3 ligases DWA1 and DWA2 can physically interact with ABI4, they do not mediate ubiquitination of ABI4. The transcription factor ZFP2 is encoded by a gene that is expressed in root tips of developing embryos and germinating seedlings which is consistent with areas of ABI4 activity. We were unable to determine if ZFP2 is involved in ABA signaling during germination or root growth, but our experiments show that ZFP2 is regulated by auxin and possibly ABA.

Physical Description:
1 online resource (76 pages)
Format:
Text
Collection(s):
UCSB electronic theses and dissertations
ARK:
ark:/48907/f3bz665j
ISBN:
9781369340426
Catalog System Number:
990047190080203776
Rights:
Inc.icon only.dark In Copyright
Copyright Holder:
Kyle Swentowsky
Access: This item is restricted to on-campus access only. Please check our FAQs or contact UCSB Library staff if you need additional assistance.